Ischemic Heart Disease - IHD


Indications for Testing

  • Patient presenting with chest pain who also has risk factors suggesting CAD
  • Clinical history and risk factor assessment

Laboratory Testing

  • Cardiac markers testing

    Guidelines – Recommended Testing Using Cardiac Markers


    • Cardiac-specific troponins have amino acid sequences different from skeletal muscle forms and therefore are highly specific for cardiac injury
    • Preferred markers for the diagnosis of acute myocardial infarction (AMI) – use in conjunction with EKG and clinical picture
      • Negative predictive value is 97-99%; lower sensitivity in AMI <6 hours old
      • Should always be used in conjunction with clinical assessment
    • Useful for distinguishing AMI from unstable angina (UA)
      • Elevated troponins indicate cardiac injury
    • Obtain blood specimen at presentation and 6-9 hours later – rising troponins confirm cardiac injury
      • Diagnosis* – maximal concentration >99th percentile of a reference control group in the first 24 hours
      • Troponins should be 5 times the 99th percentile during the first 72 hours following coronary artery bypass graft (CABG)
        • To confirm coronary artery disease (CAD) or related AMI
    • Troponins can remain elevated 10-14 days after an event

    Creatine kinase-MB isoenzyme (CK-MB)

    • Acceptable marker when troponins unavailable; useful if autoantibodies to troponins are known to exist
    • Diagnosis – maximal concentration >99th percentile of a reference control group in two successive samples or a maximal value >twice the upper limit of normal during the first 24 hours
      • Loss of specificity after 24 hours
    • Sensitivity
      • Decreased sensitivity in
        • Organ injury (acute or chronic muscle, intestine, diaphragm, uterus and prostate damage)
        • Nonspecific elevations
      • Low sensitivity in
        • Early AMI (<6 hours)
        • Late AMI (>36 hours)
        • Minimal damage AMI


    • Not recommended as a standalone test for diagnosis of AMI
      • Low specificity, high sensitivity in AMI early detection

    Natriuretic peptides (NP)

    • Atrial (ANP), brain or b-type (BNP) and N-terminal (Nt) metabolic peptides are released by cardiocytes
      • Release stimulated by ventricular wall stress
      • Function as powerful diuretics/natriuretics and as vascular smooth muscle relaxers
    • Elevated in conditions characterized by wall stretch, ventricular dilation or increased pressure
    • Most frequently used to diagnose heart failure
      • BNP measures may give additional prognostic information about mortality during first cardiovascular events

    Serial sampling of cardiac markers and repeated EKG tracings over 24 hours to rule out AMI

    *Guidelines include European Society of Cardiology, American College of Cardiology Foundation, American Heart Association, National Academy of Clinical Biochemistry, ESC/American College of Cardiology

  • Newer markers (diagnostic significance not proven)
    • Oxidative stress – lipoprotein-associated phospholipase A2, myeloperoxidase
    • Tissue necrosis – C-reactive protein, interleukin 6, other interleukins, fatty acid binding proteins, free fatty acid unbound to albumin

Imaging Studies

  • Stress testing
  • Echocardiography – wall motion abnormalities suggest AMI

Other Testing

  • Electrocardiogram (EKG) – may have false negatives
    • Typically demonstrates ST elevation, but not typically posterior MI, right ventricular infarction


  • Use of TIMI (thrombolysis in myocardial infarction) risk score prognosticates 2-week, all-cause mortality in new or recurrent AMI
    • One point for each of the following
      • Age >65 years
      • History of diabetes mellitus, hypertension, angina
      • Documented coronary stenosis >50%
      • ST elevation on EKG
      • >2 anginal events in preceding 24 hours
      • Acetylsalicylic acid (ASA) treatment in previous 7 days
      • Increased cardiac markers (troponins preferred)
      • Prior AMI, congestive heart failure (CHF), bypass surgery, or percutaneous angioplasty
    • 1 point = 5%; 2 points = 8%; 3 points = 13%; 4 points = 20%; 5 points = 26%; ≥6 points = 41%

Differential Diagnosis


  • EKG, exercise treadmill, and electron beam computerized tomography may provide prognostic information about future events
    • Screening of asymptomatic patients is not recommended (U.S. Preventive Services Task Force)

Clinical Background

Patients with ischemic heart disease (IHD) fall into 2 groups: stable angina secondary to ischemic heart disease (coronary artery disease [CAD]) and acute coronary syndromes (ACS). ACS is further grouped into acute myocardial infarction (AMI) and unstable angina (UA). CAD is the leading cause of death in the U.S.


  • Incidence – >1,500,000 cases of ACS annually in U.S.
  • Age – peak onset is >50 years
  • Sex – M>F

Risk Factors


  • Atherosclerosis – disease of large and medium-sized arteries
  • Clot formation in the coronary arteries – ACS caused by rupture or erosion of plaques
    • Rupture of plaques leads to inadequate circulation with ischemia, resulting in myocardial cell death

Clinical Presentation

  • ACS
    • Substernal chest pain, dyspnea, gastric discomfort, diaphoresis, tachycardia or hypotension
      • Atypical pain site presentations are not uncommon – arm, back, jaw, neck
    • May auscultate S3 or S4, new murmur, pericardial friction rub or bibasilar rales
    • May be difficult to distinguish the chest pain of AMI or UA from other conditions such as gastroesophageal reflux disease (GERD)
  • CAD
    • May be asymptomatic
    • May present with symptoms similar to ACS or AMI
  • Sudden death event
    • Caused by ventricular fibrillation, ventricular tachycardia, electromechanical dissociation, and bradycardias
    • Most patients have underlying CAD or structural heart disease
    • High fatality rate associated with this event

Indications for Laboratory Testing

  • Tests generally appear in the order most useful for common clinical situations
  • Click on number for test-specific information in the ARUP Laboratory Test Directory
Test Name and Number Recommended Use Limitations Follow Up
Troponin I 0090613
Method: Chemiluminescent Immunoassay
First-line laboratory test for myocardial injury

False-positive results may occur in acute pulmonary embolism, acute and chronic heart failure, sepsis, stroke, renal failure

Troponin T 0098803
Method: Quantitative Electrochemiluminescent Immunoassay
First-line laboratory test for myocardial injury

False-positive results may occur in acute pulmonary embolism, acute and chronic heart failure, sepsis, stroke, renal failure

Lower early sensitivity than troponin I for myocardial necrosis

Creatine Kinase, MB 0080480
Method: Chemiluminescent Immunoassay

Cardiac injury marker when troponins are not available

Troponins preferred

Additional Tests Available
Click the plus sign to expand the table of additional tests.
Test Name and NumberComments
Creatine Kinase, Total, Serum or Plasma 0020010
Method: Quantitative Enzymatic
Myoglobin, Serum 0020224
Method: Quantitative Electrochemiluminescent Immunoassay

May be used selectively by clinicians to evaluate causes of non-cardiac muscle injury

B-Type Natriuretic Peptide 0030191
Method: Quantitative Chemiluminescent Immunoassay
proBrain Natriuretic Peptide, NT 0050083
Method: Quantitative Electrochemiluminescent Immunoassay
Lipoprotein-Associated Phospholipase A2 (PLAC) 0081055
Method: Quantitative Enzyme-Linked Immunosorbent Assay
C-Reactive Protein 0050180
Method: Quantitative Immunoturbidimetry

Detects inflammatory processes

Interleukin 6 0051537
Method: Quantitative Multiplex Bead Assay