Qualitative Methylation-Specific Multiplex Ligation-Dependent Probe Amplification (MS-MLPA)
Beckwith-Weidemann syndrome (BWS) is a congenital overgrowth condition associated with neonatal hypoglycemia, macroglossia, macrosomia, hemihypertrophy, and an increased risk for embryonal tumors. Russell-Silver syndrome (RSS) is a congenital condition characterized by pre- and postnatal growth deficiency, limb length asymmetry, relative macrocephaly at birth, feeding difficulties, and developmental delay. Laboratory testing can confirm a suspected clinical diagnosis of BWS or RSS.
Disease Overview
Incidence
BWS: ~1/10,000-13,700 newborns
RSS: ~1/30,000-100,000 newborns
Symptoms
Genetics
Etiology
Causes of BWS
- 50% have loss of maternal methylation on chromosome 11p15 imprinting center (IC)2
- 20% have paternal uniparental disomy (UPD) for chromosome 11p15
- 5% have gain of methylation in maternal IC1
- Pathogenic sequence variants in CDKN1C
- <1% cytogenetic abnormalities involving 11p15
Causes of RSS
- 35-67% have hypomethylation of paternal IC1
- 7-10% have maternal UPD of chromosome 7
- Approximately 40% have an unknown genetic mechanism
Inheritance
- 85% of BWS cases are sporadic.
- 15% of BWS cases are inherited.
- Most cases of molecularly confirmed RSS are sporadic.
Penetrance
- Complete for RSS
- Incomplete for BWS due to methylation (e.g., individuals with a paternally inherited CDKN1C pathogenic variant will not show features of BWS)
Test Interpretation
Clinical sensitivity/specificity: 75% for BWS; 35-67% for RSS
Analytic sensitivity/specificity: 99%
Results
| Result | BWS | RSS |
|---|---|---|
| Positive | IC2 hypomethylation AND normal IC1 methylation IC1 hypermethylation AND hypomethylation of IC2 IC1 hypermethylation AND normal methylation of IC2 | IC1 hypomethylation |
| Negative | Normal methylation patterns:
| Normal methylation patterns:
|
Limitations
Molecular mechanisms causing BWS or RSS that do not affecting methylation patterns are not assessed, including:
- Maternal UPD of chromosome 7
- Chromosomal translocations, inversions, deletions, or duplications
- Pathogenic CDKN1C sequence variants, deletions/duplications
Diagnostic errors can occur due to rare sequence variations, and low-level mosaicism may not be identified.
References
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Genereviews - Beckwith-Wiedemann syndrome
Shuman C, Kalish JM, Weksberg R. Beckwith-Wiedemann syndrome. In: Adam MP, Bick S, Mirzaa GM, et al, eds. GeneReviews. University of Washington, Seattle. Updated Sep 2023; accessed Jun 2026.
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Genereviews - Silver-Russell syndrome
Saal HM, Harbison MD, Netchine I. Silver-Russell syndrome. In: Adam MP, Bick S, Mirzaa GM, et al, eds. GeneReviews. University of Washington, Seattle. Updated Jan 2025; accessed Jun 2026.
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Romanelli V, Meneses HN, Fernández L, et al. Beckwith-Wiedemann syndrome and uniparental disomy 11p: fine mapping of the recombination breakpoints and evaluation of several techniques. Eur J Hum Genet. 2011;19(4):416-421.

