Neuromyelitis Optica

Aquaporin-4 Receptor Antibody 2003036
Method: Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Aid in evaluation of NMO and NMOSD

Aquaporin-4 Receptor Antibody, IgG by IFA with Reflex to Titer, Serum 2013320
Method: Semi-Quantitative Indirect Fluorescent Antibody

Useful for initial evaluation of NMOSD

Aquaporin-4 Receptor Antibody by ELISA with Reflex to Aquaporin-4 Receptor Antibody, IgG by IFA 2013327
Method: Semi-Quantitative Enzyme-Linked Immunosorbent Assay/ Semi-Quantitative Indirect Fluorescent Antibody
  • For evaluation of optic neuritis, acute myelitis, spinal cord lesions, or autoimmune encephalitis
  • Useful in the interpretation of low-positive ELISA results when suspicion for disease is low or questionable
  • ELISA is less subjective than CBA by IFA and has comparable diagnostic performance, with a slight increase in sensitivity
  • CBA by IFA may provide additional support of a positive ELISA result
  • If CBA by IFA and ELISA are both positive, ELISA is preferred method for monitoring patients over time, as it is less subjective
Aquaporin-4 Receptor Antibody, IgG by IFA, CSF with Reflex to Titer 2011699
Method: Semi-Quantitative Indirect Fluorescent Antibody

Use in conjunction with serum autoantibody tests to diagnose NMO

Useful for initial evaluation of central nervous system (CNS) demyelinating disease or autoimmune encephalitis

Autoimmune CNS Demyelinating Disease Reflexive Panel 3001283
Method: Semi-Quantitative Indirect Fluorescent Antibody

Useful for initial evaluation of inflammatory CNS demyelinating disease, including NMOSD and NMOSD-like disorders, or autoimmune encephalitis

Neuromyelitis optica spectrum disorders (NMOSD) are rare relapsing autoimmune disorders that cause inflammation specifically in the optic nerve and spinal cord. Aquaporin-4 receptor (AQP4) and myelin oligodendrocyte glycoprotein (MOG) antibody testing is used for diagnosis and evaluation of neuromyelitis optica (NMO), acute myelitis, spinal cord lesions, autoimmune encephalitis, or NMOSD.

Disease Overview


Acute transverse myelitis (TM) – 1-4/100,000

  • <1% is NMOSD
    • Female:male, 5:1 for relapsing NMOSD associated with AQP4 antibodies
    • Gender ratio closer to 1:1 for MOG antibodies


  • Ophthalmic – ocular pain, visual disturbances, optic neuritis
  • Neurological – symmetrical para- or quadriparesis, bowel and bladder dysfunction

Diagnostic Issues

  • NMOSD is often mistaken for multiple sclerosis (MS)
  • Individuals with NMOSD have a worse prognosis
  • Treatment differs between NMOSD and MS
    • NMOSD – immunosuppressive therapy or plasmapheresis
    • MS – immune-modulation therapy
      • Corticosteroids administered only during periods of worsening inflammation


  • Neuromyelitis optica-specific immunoglobulin (NMO-IgG) recognizes the water-channel protein AQP4
  • Presence of AQP4 antibody is important in the differential diagnosis of NMOSD from other TM diseases
    • ~75% of patients with NMO express antibodies to the AQP4 receptor
    • A subset of patients with NMOSD who are seronegative for AQP4 antibodies express antibodies to MOG
  • MOG antibody is found in a subset of patients with NMOSD, including optic neuritis and TM, brainstem encephalitis, and acute disseminated encephalomyelitis (ADEM)
    • Persistence of antibody positivity may be associated with a relapsing course
  • TM disorders
    • MS
    • NMO
    • ADEM
    • Optic spinal MS (OSMS)
    • Longitudinally extensive spinal cord lesions/TM (LESCL/LETM)
    • Acute complete TM (ACTM)
    • Acute partial TM (APTM)
  • Differentiated from other TM disorders
    • Clinical course (monophasic or relapsing)
    • The presence and extent of lesions evident with magnetic resonance imaging (MRI)
      • Spinal cord
      • Brain
    • Accompanying presence of optic nerve inflammation (optic neuritis)
    • Presence of AQP4 or MOG autoantibodies

Diagnostic Criteria

Required for diagnosis of NMOSD 

  • NMOSD with AQP4-IgG
    • At least one core clinical characteristic
    • Positive for AQP4-IgG (cell-based assay by IFA or FACS preferred)
    • Exclusion of alternative diagnoses
  • NMOSD without AQP4-IgG (negative or unknown)
    • At least two core clinical characteristics associated with one or more clinical attacks meeting the following criteria:
      • Presence of at least one of the first three core clinical characteristics (if myelitis, one characteristic should be LETM)
      • Dissemination in location (at least two different core clinical characteristics)
      • MRI findings consistent with respective core clinical characteristics
    • Negative for AQP4-IgG (or testing unavailable)
    • Exclusion of alternative diagnoses
  • Core clinical characteristics
    • Optic neuritis
    • Acute myelitis
    • Area postrema syndrome (episode of otherwise unexplained intractable nausea and vomiting or hiccups)
    • Acute brainstem syndrome
    • Symptomatic diencephalic clinical syndrome with NMOSD-typical MRI lesions or narcolepsy
    • Symptomatic cerebral syndrome with NMOSD-typical brain lesions

Test Interpretation  


AQP4 Antibody

  • When criteria are met
    • Clinical sensitivity – 76% for NMO
    • Clinical specificity – 94% for NMO
  • AQP4 antibody detection by ELISA compared to IFA
    • Analytical sensitivity – 97%
    • Analytical specificity – 96.3%
  • Detection of AQP4-IgG by IFA compared to ELISA
    • Analytical sensitivity – 91%
    • Analytical specificity – 99%
  • Overall agreement between ELISA and IFA detection methods – 96%

MOG Antibody

  • Detection of MOG-IgG by IFA compared to FACS
    • Analytical sensitivity – 90.9% (10/11; one low-positive FACS specimen was negative by IFA)
    • Analytical specificity – 100%
    • Overall agreement between IFA and FACS detection methods – 98.8%



AQP4 Antibody
  • AQP4 receptor antibody – ≥3 U/mL
  • AQP4 receptor antibody with reflex – antibody detected and titered
  • AQP4 receptor antibody, cerebrospinal fluid (CSF), with reflex – antibody detected and titered
MOG Antibody
  • MOG antibody with reflex – antibody detected and titered


AQP4 Antibody
  • AQP4 receptor antibody – ≤3 U/mL
  • AQP4 receptor antibody, serum, with reflex – <1:10
  • AQP4 receptor antibody, CSF, with reflex – <1:1
MOG Antibody
  • MOG antibody, with reflex – <1:10


  • Absence of antibodies to the AQP4 receptor or MOG does not rule out a diagnosis of NMOSD
  • A negative result can occur in the setting of immunosuppression therapy
  • Testing by ELISA is not a suitable method for detecting AQP4 antibodies in CSF
  • Test performance may vary due to differences in methods and/or new versus established disease states
  1. Wingerchuk DM, Banwell B, Bennett JL, Cabre P, Carroll W, Chitnis T, de Seze J, Fujihara K, Greenberg B, Jacob A, Jarius S, Lana-Peixoto M, Levy M, Simon JH, Tenembaum S, Traboulsee AL, Waters P, Wellik KE, Weinshenker BG, International Panel for NMO Diagnosis. International consensus diagnostic criteria for neuromyelitis optica spectrum disorders. Neurology. 2015; 85(2): 177-89. PubMed
  2. Jarius S, Ruprecht K, Kleiter I, Borisow N, Asgari N, Pitarokoili K, Pache F, Stich O, Beume L, Hümmert MW, Trebst C, Ringelstein M, Aktas O, Winkelmann A, Buttmann M, Schwarz A, Zimmermann H, Brandt AU, Franciotta D, Capobianco M, Kuchling J, Haas J, Korporal-Kuhnke M, Lillevang ST, Fechner K, Schanda K, Paul F, Wildemann B, Reindl M, in cooperation with the Neuromyelitis Optica Study Group (NEMOS). MOG-IgG in NMO and related disorders: a multicenter study of 50 patients. Part 1: Frequency, syndrome specificity, influence of disease activity, long-term course, association with AQP4-IgG, and origin. J Neuroinflammation. 2016; 13(1): 279. PubMed

Last Update: June 2019