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ARUP Free or Bioavailable Testosterone Test Options (Adult Males or Individuals on Testosterone Hormone Therapy)

Free or bioavailable testosterone tests may provide supportive information after a total testosterone test. The selection of a test may be based on clinical judgment and preference.

These tests are not suitable when low testosterone concentrations, such as those found in children and cisgender females, are expected. For suitable testing options, refer to the Free or Bioavailable Testosterone Test Options (Adult Females, Children, or Individuals on Testosterone-Suppressing Hormone Therapy) table.

	Method				Results Reported
Test Name and Number	IA	MS	ED	Calc	Total T	Free T	% Free T	Bio T	SHBG
Testosterone, Free (Adult Males or Individuals on Testosterone Hormone Therapy) 0070111	✔			✔		✔
Testosterone, Free, by Dialysis and Mass Spectrometry (Adult Males or Individuals on Testosterone Hormone Therapy)^a 2003246		✔	✔			✔
Testosterone, Free and Total, Includes Sex Hormone-Binding Globulin (Adult Males or Individuals on Testosterone Hormone Therapy) 0070109	✔			✔	✔	✔	✔		✔
Testosterone, Free and Total, by Dialysis and Mass Spectrometry (Adult Males or Individuals on Testosterone Hormone Therapy)^a 2004246		✔	✔		✔	✔
Testosterone, Bioavailable and Total, Includes Sex Hormone-Binding Globulin (Adult Males or Individuals on Testosterone Hormone Therapy) 0070102	✔			✔	✔	✔	✔	✔	✔
^aLaboratory reference method for the direct measurement of free testosterone. Not recommended for the general evaluation of testosterone status. Bio, bioavailable; Calc, calculation; ED, equilibrium dialysis; IA, immunoassay; MS, mass spectrometry; SHBG, sex hormone-binding globulin; T, testosterone

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ARUP Free or Bioavailable Testosterone Test Options (Adult Males or Individuals on Testosterone Hormone Therapy)