Testing for Treponema pallidum, the causative agent for syphilis, can be performed using either the traditional method or the reverse sequence screening method. An initial workup for syphilis includes either a nontreponemal screening assay followed by a confirmatory treponemal test (traditional sequence), or an automated treponemal test followed by a confirmatory nontreponemal test (reverse sequence). In the reverse sequence, discordant results must be confirmed by another treponemal test.
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